Gene Reporter Systems
Promega Beta-Glo™ Assay System
The Beta-Glo™ Assay System is a homogeneous, luminescence-based method for quantitating beta-galactosidase expression in mammalian cells.
Promega ViviRen™ Live Cell Substrate
ViviRen Live Cell Substrate is a uniquely engineered coelenterazine that generates three- to fivefold brighter Renilla luciferase luminescence for super-sensitive quantitation of reporter gene, BRET and RNAi activity.
Promega Proteasome-Glo™ Bioluminescent Enzyme Assays
The Proteasome-Glo Assays are homogeneous, luminescent assays that individually measure the chymotrypsin-like, trypsin-like and caspase-like protease activities associated with the proteasome in a purified enzyme-based format (not suitable for cell-based applications).
Promega Luciferase Reporter Assays to Study Nuclear Receptors: pBIND-ERα Vector
NR ligand binding domain fused to yeast GAL4 TF DBD in the FN26A (BIND) Flexi Vector or use pBIND-Er∝ and pBIND-GR Vectors. Fusion protein NR activates luc2P reporter controlled by 9X GAL4 UAS in pGL4.35. Use pGL4.36 MMTV LTR for androgen or GC responses.
Promega Ultra Pure UDP-GalNAc, 100mM, 50microl
UDP-sugar substrates are used with the UDP-Glo Glycosyltransferase Assay to detect the activity of glycosyltransferases that use UDP-sugars as donor substrates and release UDP as a product.
Promega UDP-Glo Glycosyltransferase Assay + UDP-Glucuronic Acid (UDP-GA), 200 assays
The UDP-Glo Glycosyltransferase Assay is a bioluminescent assay for detecting the activity of glycosyltransferases that use UDP-sugars as donor substrates and release UDP as a product.
Promega Ultra Pure UDP-GalNAc, 100mM, 250microl
UDP-sugar substrates are used with the UDP-Glo Glycosyltransferase Assay to detect the activity of glycosyltransferases that use UDP-sugars as donor substrates and release UDP as a product.
Promega HER4 Kinase Enzyme System
Recombinant human HER4 (amino acids 682-993) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. HER4 or ERBB4 is a transmembrane receptor tyrosine kinase that belongs to the epidermal growth factor receptor family.
Promega SRC Kinase Enzyme System
Recombinant full-length human SRC was expressed in E. coli cells using an N-terminal GST tag. SRC was originally identified as a transforming protein of the Rous sarcoma virus that had enzymatic ability to phosphorylate tyrosine in protein substrates.
Promega GSK3α Kinase Enzyme System
Recombinant full-length human GSK3 alpha was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. GSK3 alpha is a multifunctional protein serine kinase implicated in the control of several regulatory proteins.
Promega cAMP-Glo™ Max Assay System
The cAMP-Glo™ Max Assay is a homogeneous, bioluminescent and high-throughput assay to measure cyclic AMP (cAMP) levels in cells. Compounds that modulate GPCRs coupled with adenylate cyclase typically alter intracellular cAMP levels.
Promega Control Ab, Anti-PD-1, 1 each
Control Ab, Anti-PD-1for use with the PD-1/PD-L1 Blockade Bioassay.
Promega ROCK1 Kinase Enzyme System
Recombinant human ROCK1 (amino acids 17-535) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. ROCK1 is a ubiquitously expressed serine-threonine kinase that is a downstream target of the small GTPase RhoA.
Promega CSK Kinase Enzyme System
Recombinant full-length human CSK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CSK has been shown to downregulate the tyrosine kinase activity of c-src through tyrosine phosphorylation of the c-src carboxy terminus.
Promega InsR Kinase Enzyme System
Recombinant human InsR (amino acids 1011-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. InsR is the insulin receptor tyrosine kinase that is involved in insulin signaling.